Compared to the vibrating sampling on the ground, there are problems of seawater damping and seawater vibrating during seabed vibrating drilling. Simplified mechanical model and modified coefficients were taken into account of the specific conditions in the seabed, the effects on vibrating penetration by various vibrating parameters of seabed vibrating sampler had been discussed in this paper. A seabed vibrating sampler suitable for seabed soil at 500 m depth had been designed according to the design theory discussed in this paper.